Florida Highway Patrol said the motorcycle struck the back of a semi-truck while turning right, shutting down southbound lanes near Wolff Road.
The highway and Veterans Memorial Bridge projects are both expected to finish before winter, with some work possibly wrapping up sooner.
Firefighters, EMS and state police responded as investigators shut down the highway and urged drivers to use alternate routes.
Fire crews are monitoring hot spots after the fast-moving blaze prompted evacuations, road closures and a state mobilization.
Authorities said the crash happened just before 5 p.m. and all lanes later reopened.
Officials said one person died and the road will remain closed while investigators examine the crash scene.
California Highway Patrol says one person died and another was taken to NorthBay Medical Center after the collision.
Troopers said the collision happened around 7:20 p.m. and urged drivers to use caution in the area.
Three people were hospitalized with non-life-threatening injuries after the midday wreck shut down both directions of the tollway.
No life-threatening injuries were reported after a pickup rear-ended traffic backed up by construction and triggered a rollover collision on I-43 North.
